Sakura Matou (間桐 桜, Matō Sakura?) is one of three main heroines of Fate/stay night and the "True" Master of Rider in Fifth Holy Grail War. She is also the Master of Avenger, Saber Alter, and the blackened Berserker in the Heaven's Feel route. Sakura is the younger, adopted sister of Shinji Matou and biological sister of Rin Tohsaka. She has a long-standing and obvious crush on Shirou Emiya, who sees her as family. After Shirou's confrontation with Shinji, Sakura would often visit Shirou's home to help him with his daily chores.

While Sakura is outwardly shy, soft-spoken and eager to please, she possesses a great inner strength. As noted by Shirou, she can also be openly stubborn at times. However, she is also prone to feelings of resentment towards others.