Ayako Mitsuzuri (美綴 綾子, Mitsuzuri Ayako?) is a character in Fate/stay night in Year 2 Class A.

She is friends with both Shirou and Rin. While she treats Rin as her friend, she treats Shirou more like a rival. The three of them haven't noticed what a delicate relationship they have with each other. Even though her room at home looks more like it belongs to a little girl than a teenager, she has no qualms about inviting Rin over to hang out. After all, it's not that she has no interest in romance, it's just that she has a thing for older men.[2] She holds the belief of a heroine that "Beauty must do martial arts".